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WB analysis of HepG2 using 23337-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 23337-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 23337-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 23337-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 23337-1-AP
IP result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug.
IHC staining of human testis using 23337-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 23337-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 23337-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 23337-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HEK-293 using 23337-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C16orf84 antibody (23337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C16orf84 Polyclonal antibody

Name: C16orf84 Polyclonal antibody
Brand: Proteintech
SKU: 23337-1-AP

C16orf84 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23337-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23337-1-AP targets C16orf84 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18428 Product name: Recombinant human C16orf84 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 165-515 aa of BC125269 Sequence: QELVGSEGAYKAAVDSFLQQQHVLGAGGGPGPTQGEEQPPQPPLDPQNLARPPAPAQTEALSQLFCSVRTLTAKEELLQTLRTHLILHMARAHGYSKVMTGDSCTRLAIKLMTNLALGRGAFLAWDTGFSDERHGDVVVVRPMRDHTLKEVAFYNRLFSVPSVFTPAVDTKAPEKASIHRLMEAFILRLQTQFPSTVSTVYRTSEKLVKGPRDGPAAGDSGPRCLLCMCALDVDAADSATAFGAQTSSRLSQMQSPIPLTETRTPPGPCCSPGVGWAQRCGQGACRREDPQACIEEQLCYSCRVNMKDLPSLDPLPPYILAEAQLRTQRAWGLQEIRDCLIEDSDDEAGQS Predict reactive species
Full Name	chromosome 16 open reading frame 84
Calculated Molecular Weight	515 aa, 56 kDa
Observed Molecular Weight	65-68 kDa
GenBank Accession Number	BC125269
Gene Symbol	C16orf84
Gene ID (NCBI)	348180
RRID	AB_2879257
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q2VPK5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.

Protocols

Product Specific Protocols
WB protocol for C16orf84 antibody 23337-1-AP	Download protocol
IHC protocol for C16orf84 antibody 23337-1-AP	Download protocol
IF protocol for C16orf84 antibody 23337-1-AP	Download protocol
IP protocol for C16orf84 antibody 23337-1-AP	Download protocol