Filter: AllWBIHCIF/ICC

WB analysis of COLO 320 using 24977-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human thyroid cancer using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 24977-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of COLO 320 using 24977-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C18orf21 Polyclonal antibody

Name: C18orf21 Polyclonal antibody
Brand: Proteintech
SKU: 24977-1-AP

C18orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24977-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells
Positive IHC detected in	human urothelial carcinoma tissue, human colon cancer tissue, human pancreas tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	COLO 320 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24977-1-AP targets C18orf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21782 Product name: Recombinant human C18orf21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-220 aa of BC119766 Sequence: MRQKHYLEAAARGLHDSCPGQARYLLWAYTSSHDDKSTFEETCPYCFQLLVLDNSRVRLKPKARLTPKIQKLLNREARNYTLSFKEAKMVKKFKDSKSVLLITCKTCNRTVKHHGKSRSFVSTLKSNPATPTSKLSLKTPERRTANPNHDMSGSKGKSPASVFRTPTSGQSVSTCSSKNTSKTKKHFSQLKMLLSQNESQKIPKVDFRNFLSSLKGGLLK Predict reactive species
Full Name	chromosome 18 open reading frame 21
Calculated Molecular Weight	220 aa, 25 kDa
Observed Molecular Weight	25-28 kDa
GenBank Accession Number	BC119766
Gene Symbol	C18orf21
Gene ID (NCBI)	83608
RRID	AB_2879829
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q32NC0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for C18orf21 antibody 24977-1-AP	Download protocol
IHC protocol for C18orf21 antibody 24977-1-AP	Download protocol
IF protocol for C18orf21 antibody 24977-1-AP	Download protocol