Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1900 Product name: Recombinant human C19orf10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC010129 Sequence: MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL Predict reactive species |
| Full Name | chromosome 19 open reading frame 10 |
| Calculated Molecular Weight | 173 aa, 19 kDa |
| Observed Molecular Weight | 15-19 kDa |
| GenBank Accession Number | BC010129 |
| Gene Symbol | MYDGF/C19orf10 |
| Gene ID (NCBI) | 56005 |
| RRID | AB_2207647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969H8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| IHC protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| WB protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MYDGF (C19orf10) polyclonal antibody (Cat# 11353-1-AP) has 14 citations published in journals including Nature Communications, Science Advances, Cell Death & Disease, Kidney International, Frontiers in Immunology, Arteriosclerosis, Thrombosis and Vascular Biology, Biofabrication, Journal of Proteome Research, Journal of Cancer, Frontiers in Oncology, and Heliyon. Associated research fields span cardiovascular disease, atherosclerosis, non-alcoholic fatty liver disease, multiple cancer types (renal, lung, bladder, mammary), glomerular disease, Sjögren's syndrome, and neuroprotection.
| Species | Application | Title |
|---|---|---|
Kidney Int Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.
| ||
Nat Commun Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes | ||
Nat Commun Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. | ||
Nat Commun Dynamic transcriptomic remodeling in grafted human neural progenitor cells uncovers mechanisms for vision preservation in a rat model of retinitis pigmentosa. | ||
Sci Adv Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.
| ||
Cell Death Dis Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling |
