Tested Applications
| Positive WB detected in | MCF-7 cells, HepG2 cells |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
26338-1-AP targets C19orf21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23712 Product name: Recombinant human C19orf21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 330-679 aa of BC052236 Sequence: LLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD Predict reactive species |
| Full Name | chromosome 19 open reading frame 21 |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC052236 |
| Gene Symbol | C19orf21 |
| Gene ID (NCBI) | 126353 |
| RRID | AB_2880482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| IF protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma. | ||
Cell Death Discov Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. | ||
J Vet Med Sci Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice
| ||
Int J Mol Sci Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway
| ||
Front Pharmacol Protein and metabolic profiles of tyrosine kinase inhibitors co-resistant liver cancer cells | ||
Adv Sci (Weinh) MISP Suppresses Ferroptosis via MST1/2 Kinases to Facilitate YAP Activation in Non-Small Cell Lung Cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH anwesh (Verified Customer) (07-29-2019) | The protein was found at the right size and no additional background bands were observed around the size range. Perfect!
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