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WB analysis using 21589-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21589-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21589-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 21589-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21589-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

C7orf10 Polyclonal antibody

Name: C7orf10 Polyclonal antibody
Brand: Proteintech
SKU: 21589-1-AP

C7orf10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21589-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Dermal papilla-derived protein 13, DERP13, EC:2.8.3.13, ORF19, Succinate--hydroxymethylglutarate CoA-transferase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse pancreas tissue, mouse skeletal muscle tissue, HepG2 cells, HEK-293 cells, rat kidney tissue
Positive IHC detected in	human kidney tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

21589-1-AP targets C7orf10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16159 Product name: Recombinant human C7orf10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 123-471 aa of BC098318 Sequence: VKIIKELAAVCDVFVENYVPGKLSAMGLGYEDIDEIAPHIIYCSITGYGQTGPISQRAGYDAVASAVSGLMHITGPENGDPVRPGVAMTDLATGLYAYGAIMAGLIQKYKTGKGLFIDCNLLSSQVACLSHIAANYLIGQKEAKRWGTAHGSIVPYQAFKTKDGYIVVGAGNNQQFATVCKILDLPELIDNSKYKTNHLRVHNRKELIKILSERFEEELTSKWLYLFEGSGVPYGPINNMKNVFAEPQNAVSGFQSLLHSLAHGPFLHLQGSARVLHNGLVMEMEHPTVGKISVPGPAVRYSKFKMSEARPPPLLGQHTTHILKEVLRYDDRAIGELLSAGVVDQHETH Predict reactive species
Full Name	chromosome 7 open reading frame 10
Calculated Molecular Weight	471 aa, 51 kDa
Observed Molecular Weight	48-55 kDa
GenBank Accession Number	BC098318
Gene Symbol	C7orf10
Gene ID (NCBI)	79783
RRID	AB_10734435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HAC7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

Protocols

Product Specific Protocols
IHC protocol for C7orf10 antibody 21589-1-AP	Download protocol
WB protocol for C7orf10 antibody 21589-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype. Authors - Joanna Niska-Blakie View Article
mouse	WB	bioRxiv Odd-chain dicarboxylic acid feeding recapitulates the biochemical phenotype of glutaric aciduria type 1 in mice Authors - Adam C Richert View Article
human	WB	Front Aging Neurosci A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations Authors - Scott P Allen View Article