Tested Applications
| Positive WB detected in | human cerebellum tissue, mouse thymus tissue |
| Positive IHC detected in | human ovary cancer tissue, human brain tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13263-1-AP targets CaMKIV in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3922 Product name: Recombinant human CAMK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-302 aa of BC025687 Sequence: MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTG Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase IV |
| Calculated Molecular Weight | 473 aa, 52 kDa |
| Observed Molecular Weight | 56-65 kDa |
| GenBank Accession Number | BC025687 |
| Gene Symbol | CAMK4 |
| Gene ID (NCBI) | 814 |
| RRID | AB_2068381 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| WB protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CaMKIV polyclonal antibody (Cat# 13263-1-AP) has 8 citations across journals including Cell Death Discovery, Journal of Investigative Dermatology, European Journal of Endocrinology, CNS Neuroscience & Therapeutics, Oncotarget, Frontiers in Molecular Biosciences, Toxicology Research, and Fitoterapia. Research fields encompass neuroscience (hippocampal spine development, Alzheimer's disease), endocrinology (aldosterone regulation), oncology (melanogenesis, microtubule dynamics), toxicology, and ischemic stroke.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV. | ||
J Invest Dermatol The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes. | ||
Eur J Endocrinol TPD52 inhibits aldosterone synthesis through suppression of CAMKK2 signaling. | ||
CNS Neurosci Ther Congenital Hypothyroidism Dysregulates TRPC6 to Mediate Abnormal Dendritic Spine Growth of Hippocampal Neurons | ||
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
Front Mol Biosci Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease |
