CAPG Antibody
Rabbit Polyclonal
Catalog number: 10194-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | HeLa cells, HL-60 cells, mouse heart tissue, mouse thymus tissue |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 10194-1-AP(CAPG Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 10194-1-AP(CAPG Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using CAPG antibody 10194-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CAPG fusion protein ag0248 | Full name | capping protein (actin filament), gelsolin-like |
|
Calculated molecular weight |
39kd |
Observed molecular
weight |
39-42kd |
|
GenBank accession number |
BC000728 | Gene ID (NCBI) | 822 |
| Gene symbol | CAPG | Synonyms | AFCP; MCP |
Background
CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129) |
