Tested Applications
| Positive WB detected in | Y79 cells, SH-SY5Y cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20766-1-AP targets CAPRIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14745 Product name: Recombinant human CAPRIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 970-1128 aa of BC007520 Sequence: MTPVDVPVTNPAATILPVHVYPLPQQMRVAFSAARTSNLAPGTLDQPIVFDLLLNNLGETFDLQLGRFNCPVNGTYVFIFHMLKLAVNVPLYVNLMKNEEVLVSAYANDGAPDHETASNHAILQLFQGDQIWLRLHRGAIYGSSWKYSTFSGYLLYQD Predict reactive species |
| Full Name | caprin family member 2 |
| Calculated Molecular Weight | 1127 aa, 126 kDa |
| Observed Molecular Weight | 126-150 kDa |
| GenBank Accession Number | BC007520 |
| Gene Symbol | CAPRIN2 |
| Gene ID (NCBI) | 65981 |
| RRID | AB_10695763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IMN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| IP protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| WB protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuroendocrinology Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2. | ||
Dev Dyn Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paul (Verified Customer) (04-29-2019) | Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.
 |
FH Criseyda (Verified Customer) (09-21-2018) | Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.
 |
