Tested Applications
| Positive WB detected in | A431 cells, mouse testis tissue, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human skin cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25043-1-AP targets CAPZB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19327 Product name: Recombinant human CAPZB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-243 aa of BC109241 Sequence: SDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTNKSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGL Predict reactive species |
| Full Name | capping protein (actin filament) muscle Z-line, beta |
| Calculated Molecular Weight | 277 aa, 31 kDa |
| Observed Molecular Weight | 30-31 kDa |
| GenBank Accession Number | BC109241 |
| Gene Symbol | CAPZB |
| Gene ID (NCBI) | 832 |
| RRID | AB_2879867 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47756 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CAPZB belongs to the F-actin-capping protein beta subunit family and is the β subunit of the heterodimeric F-actin cap-binding protein. F-actin-capping proteins bind in a Ca2+-independent manner to the fast-growing ends of actin filaments, thereby blocking the exchange of subunits at these ends.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPZB antibody 25043-1-AP | Download protocol |
| IHC protocol for CAPZB antibody 25043-1-AP | Download protocol |
| WB protocol for CAPZB antibody 25043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Rep Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.
| ||
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.
| ||
Development Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. | ||
Cell Rep Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction. | ||
Cell Rep The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice. | ||
Mol Med Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy |
