CASP10 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100 |
IP = Immunoprecipitation
HeLa cells were subjected to SDS PAGE followed by western blot with 14311-1-AP(CASP10 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human endometrial cancer using 14311-1-AP(CASP10 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human endometrial cancer using 14311-1-AP(CASP10 Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CASP10 antibody 14311-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | CASP10 fusion protein ag5511 | Full name | caspase 10, apoptosis-related cysteine peptidase |
|
Calculated molecular weight |
59kd |
Observed molecular
weight |
59kd |
|
GenBank accession number |
BC042844 | Gene ID (NCBI) | 843 |
| Gene symbol | CASP10 | Synonyms | ALPS2; FLICE2; MCH4 |
CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full lengthe has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing. This antibody is specific to CASP10. |
