CBLB Polyclonal antibody

Name: CBLB Polyclonal antibody
Brand: Proteintech
SKU: 12781-1-AP

CBLB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12781-1-AP

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12781-1-AP
IP result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:300) with HEK-293 cells lysate 3680ug.
IHC staining of mouse colon using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 10x lens)..
IHC staining of mouse colon using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 40x lens)..
IHC staining of human lung using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A431 using 12781-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBLB antibody (12781-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12781-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, A549 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse colon tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 2 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

12781-1-AP targets CBLB in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CBLB fusion protein Ag3491
Full Name	Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Calculated Molecular Weight	982 aa, 109 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC032851
Gene Symbol	CBLB
Gene ID (NCBI)	868
RRID	AB_2275326
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CBLB antibody 12781-1-AP	Download protocol
IHC protocol for CBLB antibody 12781-1-AP	Download protocol
IF protocol for CBLB antibody 12781-1-AP	Download protocol
IP protocol for CBLB antibody 12781-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Exp Med Cbl-b deficiency prevents functional but not phenotypic T cell anergy. Authors - Trang T T Nguyen View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
rat	IHC	Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. Authors - Zi-Yang Zhang View Article
human	FC	J Immunol A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function. Authors - Klarissa Hanja Stürner View Article
mouse	WB,IF	J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. Authors - Ze-Qing Pu View Article
mouse	WB	Biomolecules miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles. Authors - Xin Yang View Article

CBLB Polyclonal antibody

CBLB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

WB analysis of HEK-293 using 12781-1-AP

WB analysis of HEK-293 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

IP experiment of HEK-293 using 12781-1-AP

IHC staining of mouse colon using 12781-1-AP

IHC staining of mouse colon using 12781-1-AP

IHC staining of human lung using 12781-1-AP

IHC staining of human lung using 12781-1-AP

IHC staining of human kidney using 12781-1-AP

IHC staining of human kidney using 12781-1-AP

IHC staining of human placenta using 12781-1-AP

IHC staining of human placenta using 12781-1-AP

IHC staining of human testis using 12781-1-AP

IHC staining of human testis using 12781-1-AP

IHC staining of human spleen using 12781-1-AP

IHC staining of human spleen using 12781-1-AP

IF Staining of A431 using 12781-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cbl-b deficiency prevents functional but not phenotypic T cell anergy.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.

A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.