Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, HeLa cells, MCF-7 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
Product Information
15579-1-AP targets CBX2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7979 Product name: Recombinant human CBX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC004252 Sequence: MEELSSVGEQVFAAECILSKRLRKGKLEYLVKWRGWSSKHNSWEPEENILDPRLLLAFQKKEHEKEVQNRKRGKRPRGRPRKLTAMSSCSRRSKLKVGGCAGYADPTSQHPLGVGGRQREGLGPSGRGWHFCQQSVPLLGKQEPPFFLSLSFCCQGPQPAESSSPPLPGASCFSLSCTPLCWVAGSNCCRQALFPPRGSLGDGKEQEACVQ Predict reactive species |
| Full Name | chromobox homolog 2 (Pc class homolog, Drosophila) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC004252 |
| Gene Symbol | CBX2 |
| Gene ID (NCBI) | 84733 |
| RRID | AB_2737362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14781 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The chromobox homologue protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX2 antibody 15579-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.
| ||
Am J Transl Res Overexpression of CBX2 in breast cancer promotes tumor progression through the PI3K/AKT signaling pathway.
| ||
Mol Carcinog Loss of CBX2 causes genomic instability and Wnt activation in high grade serous ovarian carcinoma cells
| ||
Oncol Lett Comprehensive analysis reveals the value of the expression of chromobox family members for bladder urothelial carcinoma prognosis | ||
Heliyon Derepressing of STAT3 and USP7 contributes to resistance of DLBCL to EZH2 inhibition |
