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MCU/CCDC109A Polyclonal antibody

MCU/CCDC109A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26312-1-AP

Synonyms

C10orf42, Calcium uniporter protein, CCDC109A, MCU, MCU/CCDC109A



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26312-1-AP is MCU/CCDC109A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCU/CCDC109A fusion protein Ag24733
Full Name coiled-coil domain containing 109A
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC034235
Gene Symbol CCDC109A
Gene ID (NCBI) 90550
RRIDAB_2880474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).

Protocols

Product Specific Protocols
WB protocol for MCU/CCDC109A antibody 26312-1-APDownload protocol
IHC protocol for MCU/CCDC109A antibody 26312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak.

Authors - Giovanni Quarato
humanWB

Cell Rep

A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease

Authors - Vinita Bharat
mouseWB

Int J Mol Sci

Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes

Authors - Tianyang Lan
humanWB

Cancer Sci

EFHD1, a novel mitochondrial regulator of tumor metastasis in clear cell renal cell carcinoma

Authors - Kun Meng
ratWB

Mol Nutr Food Res

Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).

Authors - Sicong Tian
ratWB

Stem Cell Res Ther

PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca2+ efflux.

Authors - Qin Zhou