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CCDC12 Polyclonal antibody

CCDC12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25138-1-AP

Synonyms

CCDC12

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CCDC12 Polyclonal antibody (25138-1-AP)

    WB analysis of HeLa using 25138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CCDC12 Polyclonal antibody (25138-1-AP)

    WB analysis of Raji using 25138-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CCDC12 Polyclonal antibody (25138-1-AP)

    IP experiment of HeLa using 25138-1-AP

    IP result of anti-CCDC12 (IP:25138-1-AP, 4ug; Detection:25138-1-AP 1:600) with HeLa cells lysate 2560ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CCDC12 Polyclonal antibody (25138-1-AP)

    IHC staining of human ovary tumor using 25138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CCDC12 Polyclonal antibody (25138-1-AP)

    IHC staining of human ovary tumor using 25138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC12 Polyclonal antibody (25138-1-AP)

    IF Staining of HeLa using 25138-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25138-1-AP (CCDC12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25138-1-AP targets CCDC12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC12 fusion protein Ag18223
Full Name coiled-coil domain containing 12
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 18-25 kDa
GenBank Accession NumberBC020830
Gene Symbol CCDC12
Gene ID (NCBI) 151903
RRIDAB_2879919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC12 is a 166aa protein with MW 18-25 kDa (with some modification).

Protocols

Product Specific Protocols
WB protocol for CCDC12 antibody 25138-1-APDownload protocol
IHC protocol for CCDC12 antibody 25138-1-APDownload protocol
IF protocol for CCDC12 antibody 25138-1-APDownload protocol
IP protocol for CCDC12 antibody 25138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols