CCDC53 Polyclonal antibody

CCDC53 Polyclonal Antibody for IHC, ELISA

Cat No. 24445-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllIHC

IHC staining of human placenta using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 24445-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (5)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human placenta tissue, human breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24445-1-AP targets CCDC53 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19831 Product name: Recombinant human CCDC53 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC010889 Sequence: MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSTVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLDDVTVEVSPLNVTSVTNGAHPEATSEQPQQSSTQDSGLQESEVSAENILTVAKDPRYARYLKMVQVGVPVMAIRNKMISEGLDPDLLERPDAPVPDGESEKTVEESSDSESSFSD Predict reactive species
Full Name	coiled-coil domain containing 53
Calculated Molecular Weight	194 aa, 21 kDa
GenBank Accession Number	BC010889
Gene Symbol	CCDC53
Gene ID (NCBI)	51019
RRID	AB_2879551
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3C0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CCDC53 antibody 24445-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Nature Architecture of the human interactome defines protein communities and disease networks. Authors - Edward L Huttlin View Article
human	WB	Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Authors - Yunkai Zhu View Article
human	WB	Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. Authors - Danqiong Liu View Article
human	WB	Int J Mol Sci Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing. Authors - You Li KO Validated View Article
zebrafish	WB	Front Cardiovasc Med WASHC3 knockout disrupts mitochondrial protein homeostasis and energy metabolism in cardiomyocytes. Authors - Sujin Kim View Article