CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 60264-1-Ig

Clone No.5F5A6

Publications(3)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

TCP 1 gamma, 5F5A6, CCT gamma, CCTG, CCTgamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHCIF/ICC

WB analysis of COLO 320 using 60264-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 60264-1-Ig (CCT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 60264-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 60264-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 60264-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (60264-1-Ig, Clone: 5F5A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	COLO 320 cells
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

60264-1-Ig targets CCT3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0843 Product name: Recombinant human CCT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 246-545 aa of BC006501 Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE Predict reactive species
Full Name	chaperonin containing TCP1, subunit 3 (gamma)
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	56-60 kDa
GenBank Accession Number	BC006501
Gene Symbol	CCT3
Gene ID (NCBI)	7203
RRID	AB_2881385
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49368
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for CCT3 antibody 60264-1-Ig	Download protocol
IHC protocol for CCT3 antibody 60264-1-Ig	Download protocol
WB protocol for CCT3 antibody 60264-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse,human	WB,IF,CoIP	Front Cell Dev Biol Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins Authors - Joshua Linnert View Article
human	IF	J Clin Invest ANKRD55 is a key regulator of T cell inflammation in multiple sclerosis Authors - Chuyu Wu View Article
human	WB	Front Cell Dev Biol The BBS/CCT chaperonin complex ensures the localization of the adhesion G protein-coupled receptor ADGRV1 to the base of primary cilia Authors - Joshua Linnert View Article