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KD/KO Validated

CCT4 Polyclonal antibody

CCT4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21524-1-AP

Publications(10)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CCTD, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, SRB, Stimulator of TAR RNA-binding

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of HEK-293 using 21524-1-AP
WB result of CCT4 antibody (21524-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT4 transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 21524-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21524-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse heart using 21524-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat colon using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 21524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (10)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue
Positive IHC detected in	human liver cancer tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21524-1-AP targets CCT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16128 Product name: Recombinant human CCT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD Predict reactive species
Full Name	chaperonin containing TCP1, subunit 4 (delta)
Calculated Molecular Weight	539 aa, 58 kDa
Observed Molecular Weight	58-60 kDa
GenBank Accession Number	BC106934
Gene Symbol	CCT4
Gene ID (NCBI)	10575
RRID	AB_10733520
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50991
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT4 (Chaperonin containing TCP1 complex 4), also known as TCP-1-delta, is a recent identified member of the family, that serves as a component of the chaperonin-containing TCP1 complex (TRiC) and also functions as a monomer. CCT4 has been shown to influence the cytoskeletal structure and regulate F-actin organization independently of TRiC (PMID: 41104595). CCT4 was highly expressed in human ESCC tissues and cell lines, and closely related to the poor prognosis (PMID: 36102200).

Protocols

Product Specific Protocols
IHC protocol for CCT4 antibody 21524-1-AP	Download protocol
WB protocol for CCT4 antibody 21524-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cell CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Authors - Xinyu Ma KD Validated View Article
human	WB	J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article
human	WB	Mol Ther Nucleic Acids hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. Authors - Qiang Luo View Article
human	WB	Pathog Dis Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT. Authors - Hirai Kimito K View Article
human	IHC	Medicine (Baltimore) Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study Authors - Ruijuan Du View Article
mouse	WB,IHC	Mol Cell PDCD5 promotes substrate release from the TRiC complex in cilia and flagella. Authors - Huafang Wei View Article