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KD/KO Validated

CCT5 Polyclonal antibody

CCT5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11603-1-AP

Publications(20)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CCT epsilon, CCTE, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5, KIAA0098

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIPIHCIF/ICC

WB analysis using 11603-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 11603-1-AP
WB result of CCT5 antibody (11603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 11603-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 11603-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 11603-1-AP
IP result of anti-CCT5 (IP:11603-1-AP, 4ug; Detection:11603-1-AP 1:5000) with HeLa cells lysate 960 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 11603-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 11603-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 11603-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 11603-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11603-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT5 antibody (11603-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11603-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT5 antibody (11603-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U2OS using 11603-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT5 antibody (11603-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (20)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, HEK-293 cells, A431 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11603-1-AP targets CCT5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2146 Product name: Recombinant human CCT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 243-541 aa of BC035499 Sequence: VEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE Predict reactive species
Full Name	chaperonin containing TCP1, subunit 5 (epsilon)
Calculated Molecular Weight	541 aa, 60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC035499
Gene Symbol	CCT5
Gene ID (NCBI)	22948
RRID	AB_2073774
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48643
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Protocols

Product Specific Protocols
IF protocol for CCT5 antibody 11603-1-AP	Download protocol
IHC protocol for CCT5 antibody 11603-1-AP	Download protocol
IP protocol for CCT5 antibody 11603-1-AP	Download protocol
WB protocol for CCT5 antibody 11603-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cell CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Authors - Xinyu Ma KD Validated View Article
mouse	WB	Nat Genet DYX1C1 is required for axonemal dynein assembly and ciliary motility. Authors - Tarkar Aarti A View Article
human	WB,IF	Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Authors - Seo Seongjin S View Article
human	WB,IF	J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article
mouse,human	WB	Front Cell Dev Biol TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis Authors - Wen-Shuai Tang View Article
chicken	WB,IF	Front Microbiol Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins. Authors - Xiaohan Zhang KD Validated View Article