CCT8 Antibody
Rabbit Polyclonal
Catalog number: 12263-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human |
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 12263-1-AP(CCT8 Antibody) at dilution of 1:1500
Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CCT8 fusion protein ag2926 | Full name | chaperonin containing TCP1, subunit 8 (theta) |
|
Calculated molecular weight |
548aa,60kd |
Observed molecular
weight |
60kd |
|
GenBank accession number |
BC012584 | Gene ID (NCBI) | 10694 |
| Gene symbol | CCT8 | Synonyms | C21orf112; Cctq; D21S246; KIAA0002; PRED71 |
Background
CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638) |
