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CD163 Polyclonal antibody

CD163 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16646-1-AP

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163



Tested Applications

Positive WB detected inpig spleen tissue, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human liver tissue, human placenta tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human liver tissue
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16646-1-AP targets CD163 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD163 fusion protein Ag10029
Full Name CD163 molecule
Calculated Molecular Weight 1156 aa, 125 kDa
Observed Molecular Weight 130-145 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
RRIDAB_2756528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Protocols

Product Specific Protocols
WB protocol for CD163 antibody 16646-1-APDownload protocol
IHC protocol for CD163 antibody 16646-1-APDownload protocol
IF protocol for CD163 antibody 16646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mil Med Res

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Authors - Guang-Hui Deng
humanIF

J Clin Invest

CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

Authors - Guohao Zhang
humanIHC

Cell Rep Med

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response

Authors - Rong Tang
humanIF

Nat Commun

Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target.

Authors - Nourhan Abdelfattah
humanIHC

EBioMedicine

Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes

Authors - Fan Wu
humanWB,IF

Clin Transl Med

Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour

Authors - Kaiyuan Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jaouhara (Verified Customer) (12-05-2023)

Works very well in immunohistochemistry (human cartilage)

  • Applications: Immunohistochemistry
  • Cell Tissue Type: Cartilage
FH

Kenzo (Verified Customer) (08-29-2023)

Works well by IF on frozen mouse liver tissue sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Liver
FH

Yasuyo (Verified Customer) (05-27-2022)

Works pretty good.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Gastric Cancer
CD163 Antibody Immunofluorescence validation (1:250 dilution) in Gastric Cancer (Cat no:16646-1-AP)
FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Prostate
CD163 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:16646-1-AP)