Tested Applications
| Positive WB detected in | Raji cells, HL-60 cells, Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25284-1-AP targets CD38 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17868 Product name: Recombinant human CD38 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-300 aa of BC007964 Sequence: MANCEFSPVSGDKPCCRLSRRAQLCLGVSILVLILVVVLAVVVPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI Predict reactive species |
| Full Name | CD38 molecule |
| Calculated Molecular Weight | 300 aa, 34 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC007964 |
| Gene Symbol | CD38 |
| Gene ID (NCBI) | 952 |
| ENSEMBL Gene ID | ENSG00000004468 |
| RRID | AB_2880007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD38 antibody 25284-1-AP | Download protocol |
| WB protocol for CD38 antibody 25284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD38 antibody (Cat# 25284-1-AP) has 17 citations across journals including Oncogene, Cell Reports, PLoS Pathogens, iScience, Journal of Immunotherapy of Cancer, Aging and Disease, Frontiers in Oncology, Advances in Materials, and others. Research fields span oncology (multiple myeloma, cervical cancer, breast cancer, hepatocellular carcinoma), immunology, neuroscience (vascular dementia, HIV-related BBB dysfunction), cardiovascular disease, gastroenterology, and wound healing. Applications include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Integrin-Mediated Mechanosensing of Modeled Lymph Node Microenvironment Promotes T Cell Activation via Nuclear Deformation. | ||
J Immunother Cancer Targeting CD74 may mitigate immune-escape features and enhance BCMA CAR-T activity in preclinical models of relapsed/refractory multiple myeloma. | ||
Cell Commun Signal TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma. | ||
Aging Dis ATF4 Contributes to Abdominal Aortic Aneurysm Formation via Modulating M1 Macrophage Polarization and Inflammation | ||
Oncogene NMRK2 leads to the depletion of CD8(+)T cells by mediating the enhancement of NAD(+)-SIRT1-CD38 axis in PRCC-TFE3 rRCC. | ||
Cell Rep Tak1 licenses mitochondrial transfer from astrocytes to POMC neurons to maintain glucose and cholesterol homeostasis |
