Tested Applications
| Positive WB detected in | Raji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10878-1-AP targets CD58 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1165 Product name: Recombinant human CD58 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC005930 Sequence: MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGMYAF Predict reactive species |
| Full Name | CD58 molecule |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC005930 |
| Gene Symbol | CD58 |
| Gene ID (NCBI) | 965 |
| RRID | AB_2260179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19256 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD58 antibody 10878-1-AP | Download protocol |
| WB protocol for CD58 antibody 10878-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 10878-1-AP (anti-CD58) has 2 total citations, appearing in Frontiers in Oncology (2025) and Heliyon (2024). Both studies focus on glioma research, examining CD58's role in the immunosuppressive tumor microenvironment and its clinical significance as an immune-related antigen. Applications include Western Blot and Immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
Front Oncol CD58 reshapes the immunosuppressive microenvironment in gliomas through PD-L1 upregulation.
| ||
Heliyon Clinical significance of immune-related antigen CD58 in gliomas and analysis of its potential core related gene clusters |
