Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13071-1-AP targets CD97 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3745 Product name: Recombinant human CD97 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-350 aa of BC026690 Sequence: QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLS Predict reactive species |
| Full Name | CD97 molecule |
| Calculated Molecular Weight | 722 aa, 79 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC026690 |
| Gene Symbol | CD97 |
| Gene ID (NCBI) | 976 |
| RRID | AB_10646477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48960 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD97 antibody 13071-1-AP | Download protocol |
| WB protocol for CD97 antibody 13071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Fiona L.) rated this product 3/5 stars in January 2020. She used it at 1:1000 dilution on multiple cell lines for Western Blot and Immunofluorescence. While the blot was clean and she observed specific staining in IF, she did not see the expected band in U-937 cells (a positive control) and noted the assay required further optimization. Overall, the experience was mixed — good specificity but some troubleshooting needed for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fiona (Verified Customer) (01-09-2020) | Used sample to screen cell lines for expression of the CD97 protein. Didn't observe band where expected for U-937 cells (positive control) however assay requires further optimisation. Otherwise blot was clean. Specific clean staining observed when used for immunofluorescence.
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