CDC25B Antibody
Rabbit Polyclonal
Catalog number: 10644-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells |
| Positive IHC detected in | human lymphoma |
| Recommended dilution | WB: 1:200-1:1000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP(CDC25B Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human lymphoma using 10644-1-AP(CDC25B Antibody) at Dilution 1:100 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CDC25B fusion protein ag0994 | Full name | cell division cycle 25 homolog B (S. pombe) |
|
Calculated molecular weight |
65kd |
Observed molecular
weight |
60-70kd |
|
GenBank accession number |
BC006395 | Gene ID (NCBI) | 994 |
| Gene symbol | CDC25B | Synonyms |
Background
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B). |
