CDC27; APC3 Antibody
Rabbit Polyclonal
Catalog number: 10918-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | K-562 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | SH-SY5Y cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of CDC27 antibody (10918-1-AP for IP and Detection) with K-562 cell lysate.
K-562 cells were subjected to SDS PAGE followed by western blot with 10918-1-AP(CDC27; APC3 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human prostate cancer using 10918-1-AP(CDC27; APC3 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CDC27; APC3 fusion protein ag1345 | Full name | cell division cycle 27 homolog (S. cerevisiae) |
|
Calculated molecular weight |
92kd |
Observed molecular
weight |
92kd |
|
GenBank accession number |
BC011656 | Gene ID (NCBI) | 996 |
| Gene symbol | CDC27 | Synonyms | APC3; CDC27Hs; D0S1430E; D17S978E; HNUC |
Background
CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle. |
