CDC27; APC3 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Species specificity||human, mouse, rat; other species not tested.|
|Positive WB detected in||K-562 cells, Jurkat cells|
|Positive IHC detected in||human prostate cancer|
|Positive IF detected in||SH-SY5Y cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- IP result of CDC27 antibody (10918-1-AP for IP and Detection) with K-562 cell lysate.
- K-562 cells were subjected to SDS PAGE followed by western blot with 10918-1-AP(CDC27; APC3 Antibody) at dilution of 1:600
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10918-1-AP(CDC27; APC3 Antibody) at Dilution 1:50 (under 10x lens)
- Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||CDC27; APC3 fusion protein ag1345||Full name||cell division cycle 27 homolog (S. cerevisiae)|
|BC011656||Gene ID (NCBI)||996|
|Gene symbol||CDC27||Synonyms||APC3; CDC27Hs; D0S1430E; D17S978E; HNUC|
CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.