CDC34 Antibody
Rabbit Polyclonal
Catalog number: 10964-2-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | human pancreas tissue, human brain tissue |
| Positive IHC detected in | human prostate cancer |
| Recommended dilution | WB: 1:200-1:1000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP(CDC34 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human prostate cancer using 10964-2-AP(CDC34 Antibody) at Dilution 1:100 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CDC34 fusion protein ag1438 | Full name | cell division cycle 34 homolog (S. cerevisiae) |
|
Calculated molecular weight |
34kd |
Observed molecular
weight |
34kd |
|
GenBank accession number |
BC009850 | Gene ID (NCBI) | 997 |
| Gene symbol | CDC34 | Synonyms | E2-CDC34; UBC3; UBE2R1 |
Background
CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody is specific to CDC34. |
