Tested Applications
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
15594-1-AP targets CDCA3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7951 Product name: Recombinant human CDCA3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-268 aa of BC002551 Sequence: MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETEDSKSNLPPEPVLPPEAPLSSELDLPLGTQLSVEEQMPPWNQTEFPSKQVFSKEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQGQDHDKENQHFPLVES Predict reactive species |
| Full Name | cell division cycle associated 3 |
| Calculated Molecular Weight | 29 kDa |
| GenBank Accession Number | BC002551 |
| Gene Symbol | CDCA3 |
| Gene ID (NCBI) | 83461 |
| RRID | AB_2878154 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99618 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDCA3, also named C8, GRCC8, and TOME1, is an F-box-like protein required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at the G2/M phase.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDCA3 antibody 15594-1-AP | Download protocol |
| IHC protocol for CDCA3 antibody 15594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. | ||
Cancer Cell Int The inhibitory effect of silencing CDCA3 on migration and proliferation in bladder urothelial carcinoma.
| ||
Naunyn Schmiedebergs Arch Pharmacol Curcumin targets miR-134-5p to suppress the progression of colorectal cancer through regulating the CDCA3/CDK1 pathway | ||
Biochem Biophys Res Commun CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer.
| ||
Pathol Res Pract miR-145-5p suppresses proliferation, metastasis and EMT of colorectal cancer by targeting CDCA3. | ||
J Oncol CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma |
