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CDCA4 Polyclonal antibody

CDCA4 Polyclonal Antibody for WB, ELISA

Cat No. 11625-1-AP

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

CDCA4, HEPP, SEI 3/HEPP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of HeLa using 11625-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11625-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 11625-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 11625-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11625-1-AP targets CDCA4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2214 Product name: Recombinant human CDCA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC025263 Sequence: MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLNRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET Predict reactive species
Full Name	cell division cycle associated 4
Calculated Molecular Weight	241 aa, 26 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC025263
Gene Symbol	CDCA4
Gene ID (NCBI)	55038
RRID	AB_2260414
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BXL8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].

Protocols

Product Specific Protocols
WB protocol for CDCA4 antibody 11625-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-CDCA4 antibody (Cat# 11625-1-AP) has 14 total citations. Publications appear in journals including Cancer Cell, Scientific Reports, Cancer Cell International, Frontiers in Molecular Biosciences, Frontiers in Oncology, Journal of Cancer Research and Clinical Oncology, Thoracic Cancer, Gene, IUBMB Life, Heliyon, Kaohsiung Journal of Medical Sciences, Cell Biochemistry and Function, International Journal of Biological Macromolecules, and Science in Progress. Research fields span lung adenocarcinoma, breast cancer, bladder cancer, B-cell leukemia, hepatocellular carcinoma, EMT, autophagy/mitophagy, and signaling pathway regulation.

Species	Application	Title
mouse	WB	Cancer Cell The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia. Authors - Ulf Klein KD Validated View Article
		Int J Biol Macromol Novel mechanism of substrate recognition by protein phosphatase PP2A:B55 involving SERTAD family adapter proteins. Authors - Koyo Yano View Article
human	WB,CoIP	Cancer Cell Int CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy. Authors - Chenxin Xu KD Validated View Article
human	IF	Sci Rep Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin. Authors - Honghai Xia View Article
human	WB	Front Mol Biosci Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients Authors - Liping Shi View Article
		Cell Biochem Funct Exosomal MicroRNA-424-5p From Polycystic Ovary Syndrome Follicular Fluid Attenuates Ovarian Inflammation via Targeting the Fibroblast Growth Factor 2/Fibroblast Growth Factor Receptor 1 Axis. Authors - Dong Yuan View Article

CDCA4 Polyclonal antibody

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WB analysis of HeLa using 11625-1-AP

WB analysis using 11625-1-AP

WB analysis of HEK-293 using 11625-1-AP

WB analysis of HeLa using 11625-1-AP

Tested Applications

Recommended dilution

Product Information

Background Information

Protocols

Publications

What published studies show

The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.

Novel mechanism of substrate recognition by protein phosphatase PP2A:B55 involving SERTAD family adapter proteins.

CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.

Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.

Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients

Exosomal MicroRNA-424-5p From Polycystic Ovary Syndrome Follicular Fluid Attenuates Ovarian Inflammation via Targeting the Fibroblast Growth Factor 2/Fibroblast Growth Factor Receptor 1 Axis.