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R-cadherin Polyclonal antibody

R-cadherin Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19795-1-AP

Synonyms

CAD4, Cadherin 4, CDH4, R CAD, RCAD, R-cadherin, Retinal cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using R-cadherin Polyclonal antibody (19795-1-AP)

    WB analysis of mouse brain using 19795-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19795-1-AP (R-cadherin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using R-cadherin Polyclonal antibody (19795-1-AP)

    WB analysis of HepG2 using 19795-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19795-1-AP (R-cadherin antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using R-cadherin Polyclonal antibody (19795-1-AP)

    WB analysis of mouse brain using 19795-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19795-1-AP (R-cadherin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19795-1-AP targets R-cadherin in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cadherin 4, type 1, R-cadherin (retinal)
Calculated Molecular Weight 100 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberNM_001794
Gene Symbol CDH4
Gene ID (NCBI) 1002
RRIDAB_10642434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

R-cadherin (CDH4) is one of cadherins which are calcium dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. It may play an important role in retinal development.

Protocols

Product Specific Protocols
WB protocol for R-cadherin antibody 19795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Aging Cell

Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.

Authors - Zhi Chen
View Article