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p35 Polyclonal antibody

p35 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18346-1-AP

Synonyms

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p35 Polyclonal antibody (18346-1-AP)

    WB analysis using 18346-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18346-1-AP (p35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using p35 Polyclonal antibody (18346-1-AP)

    IP experiment of mouse brain using 18346-1-AP

    IP result of anti-p35 (IP:18346-1-AP, 4ug; Detection:18346-1-AP 1:500) with mouse brain tissue lysate 3440ug.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

18346-1-AP targets p35 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p35 fusion protein Ag13152
Full Name cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC020580
Gene Symbol CDK5R1
Gene ID (NCBI) 8851
RRIDAB_2878535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.

Protocols

Product Specific Protocols
WB protocol for p35 antibody 18346-1-APDownload protocol
IP protocol for p35 antibody 18346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Extracellular CIRP Induces Calpain Activation in Neurons via PLC-IP3-Dependent Calcium Pathway

Authors - Archna Sharma
View Article
mouseWB

Neuropharmacology

Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Authors - Yue Li
View Article
mouseWB

Front Aging Neurosci

The γ-Adducin 1-357 fragment promotes tau pathology

Authors - Honglu Yu
View Article