CEBPZ Polyclonal antibody

CEBPZ Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25612-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CBF, CBF2, CCAAT binding factor, CCAAT/enhancer-binding protein zeta, CCAAT-binding factor

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICC

WB analysis using 25612-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 25612-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 25612-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 25612-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 25612-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 25612-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 25612-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, Jurkat cells, K-562 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25612-1-AP targets CEBPZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11003 Product name: Recombinant human CEBPZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 705-1054 aa of BC034475 Sequence: FCGAENTSLWELKKLSVHFHPSVALFAKTILQGNYIQYSGDPLQDFTLMRFLDRFVYRNPKPHKGKENTDSVVMQPKRKHFIKDIRHLPVNSKEFLAKEESQIPVDEVFFHRYYKKVAVKEKQKRDADEESIEDVDDEEFEELIDTFEDDNCFSSGKDDMDFAGNVKKRTKGAKDNTLDEDSEGSDDELGNLDDDEVSLGSMDDEEFAEVDEDGGTFMDVLDDESESVPELEVHSKVSTKKSKRKGTDDFDFAGSFQGPRKKKRNLNDSSLFVSAEEFGHLLDENMGSKFDNIGMNAMANKDNASLKQLRWEAERDDWLHNRDAKSIIKKKKHFKKKRIKTTQKTKKQRK Predict reactive species
Full Name	CCAAT/enhancer binding protein (C/EBP), zeta
Calculated Molecular Weight	1054 aa, 121 kDa
Observed Molecular Weight	120-130 kDa
GenBank Accession Number	BC034475
Gene Symbol	CEBPZ
Gene ID (NCBI)	10153
RRID	AB_2880160
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q03701
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

Protocols

Product Specific Protocols
IF protocol for CEBPZ antibody 25612-1-AP	Download protocol
WB protocol for CEBPZ antibody 25612-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cancer Sci CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation. Authors - Hanxiao Shi View Article
mouse	WB	Mol Cell Proteomics Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression. Authors - Tong Wu View Article