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CES7-Specific Polyclonal antibody

CES7-Specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 19520-1-AP

Synonyms

Carboxylesterase 7, CAUXIN, CES4C1, CES5, CES7

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CES7-Specific Polyclonal antibody (19520-1-AP)

    WB analysis of HepG2 using 19520-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19520-1-AP (CES7-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CES7-Specific Polyclonal antibody (19520-1-AP)

    IF Staining of HepG2 using 19520-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19520-1-AP (CES7-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19520-1-AP targets CES7-Specific in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name carboxylesterase 7
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 58-60 kDa
GenBank Accession NumberNM_145024
Gene Symbol CES7
Gene ID (NCBI) 221223
RRIDAB_10642002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES7, also named as CAUXIN, CES4C1, CES5 and FLJ31547, belongs to the type-B carboxylesterase/lipase family. CES7 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES7 catalyze the reaction: A carboxylic ester + H2O = an alcohol + a carboxylate. The antibody can recognize the isoform1 and isoform2.

Protocols

Product Specific Protocols
WB protocol for CES7-Specific antibody 19520-1-APDownload protocol
IF protocol for CES7-Specific antibody 19520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols