Tested Applications
| Positive WB detected in | fetal human brain tissue, rat brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15877-1-AP targets Centrin 2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8638 Product name: Recombinant human Centrin 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-172 aa of BC005334 Sequence: MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY Predict reactive species |
| Full Name | centrin, EF-hand protein, 2 |
| Calculated Molecular Weight | 172 aa, 20 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC005334 |
| Gene Symbol | Centrin 2 |
| Gene ID (NCBI) | 1069 |
| RRID | AB_2077383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41208 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Centrin 2 is also named as CALT, or CEN2. It plays a fundamental role in microtubule-organizing center structure and function. Oxidizing radiation induces polymerization of centrin 2 into dimers, tetramers, hexamers,and even higher molecular mass species, as a function of the radiation dose(PMID:17603931).It exsits as a 21-kD polypeptide specifically localized to the centrosome of interphase and mitotic cells in both HeLa and BHK cells (PMID:8248209).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Centrin 2 antibody 15877-1-AP | Download protocol |
| IP protocol for Centrin 2 antibody 15877-1-AP | Download protocol |
| WB protocol for Centrin 2 antibody 15877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Centrin 2 Polyclonal antibody (Cat# 15877-1-AP) has 19 citations published in journals including Cell, Cell Research, Science Bulletin, JCI, PNAS, EMBO Journal, Genes & Development, PLoS Biology, Neurobiology of Disease, J Cell Sci, Cell Cycle, eLife, and bioRxiv. Research fields span DNA repair, centrosome/ciliogenesis biology, oocyte development, stem cell biology, retinal disease, Huntington's disease, cell cycle regulation, nuclear receptor signaling, and RNA processing.
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
J Clin Invest USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis. | ||
Proc Natl Acad Sci U S A Functional and mechanistic studies of XPC DNA-repair complex as transcriptional coactivator in embryonic stem cells. | ||
