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  • Featured Product
  • KD/KO Validated

CHAC2 Polyclonal antibody

CHAC2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16304-1-AP

Synonyms

CHAC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHAC2 Polyclonal antibody (16304-1-AP)

    WB analysis using 16304-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16304-1-AP (CHAC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CHAC2 Polyclonal antibody (16304-1-AP)

    IF Staining of MCF-7 using 16304-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CHAC2 antibody 16304-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inJurkat cells, Raji cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16304-1-AP targets CHAC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHAC2 fusion protein Ag9281
Full Name ChaC, cation transport regulator homolog 2 (E. coli)
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC019239
Gene Symbol CHAC2
Gene ID (NCBI) 494143
RRIDAB_2229405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHAC2 antibody 16304-1-APDownload protocol
IF protocol for CHAC2 antibody 16304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Cells

Adh Promotes Actinobacillus pleuropneumoniae Survival in Porcine Alveolar Macrophages by Inhibiting CHAC2-Mediated Respiratory Burst and Inflammatory Cytokine Expression

Authors - Junhui Zhu
View Article
mouseWB

Environ Toxicol

Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.

Authors - Xiaohan Zhai
  • KD Validated
View Article
human,mouseWB,IHC

J Cancer

CHAC2 promotes lung adenocarcinoma by regulating ROS-mediated MAPK pathway activation

Authors - Weilin Peng
  • KO Validated
View Article