CHD4 Antibody
Rabbit Polyclonal
Catalog number: 14173-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IF detected in | A549 cells |
| Recommended dilution | WB: 1:200-1:2000 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 14173-1-AP(CHD4 Antibody) at dilution of 1:500
Immunofluorescent analysis of A549 cells, using CHD4 antibody 14173-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CHD4 fusion protein ag5372 | Full name | chromodomain helicase DNA binding protein 4 |
|
Calculated molecular weight |
1937aa,221kd |
Observed molecular
weight |
260kd |
|
GenBank accession number |
BC038596 | Gene ID (NCBI) | 1108 |
| Gene symbol | CHD4 | Synonyms | DKFZp686E06161; Mi-2b; Mi2-BETA |
Background
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. |
