CHD4 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IF|
|Species specificity||human, mouse, rat; other species not tested.|
|Positive WB detected in||HeLa cells, HEK-293 cells|
|Positive IF detected in||A549 cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- HeLa cells were subjected to SDS PAGE followed by western blot with 14173-1-AP(CHD4 Antibody) at dilution of 1:500
- Immunofluorescent analysis of A549 cells, using CHD4 antibody 14173-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||CHD4 fusion protein ag5372||Full name||chromodomain helicase DNA binding protein 4|
|BC038596||Gene ID (NCBI)||1108|
|Gene symbol||CHD4||Synonyms||DKFZp686E06161; Mi-2b; Mi2-BETA|
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182].