CIRBP Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Y79 cells, HEK-293 cells, A549 cells, HepG2 cells, human brain tissue, mouse brain tissue, mouse testis tissue |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
IP result of anti-CIRBP (10209-2-AP for IP and Detection) with HepG2 cell lysate.
Y79 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP(CIRBP Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer using 10209-2-AP(CIRBP Antibody) at Dilution 1:100 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | CIRBP fusion protein ag0280 | Full name | cold inducible RNA binding protein |
|
Calculated molecular weight |
172aa, 19 kDa |
Observed molecular
weight |
19kd |
|
GenBank accession number |
BC000403 | Gene ID (NCBI) | 1153 |
| Gene symbol | CIRBP | Synonyms | CIRP |
CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin. |
