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  • Featured Product
  • KD/KO Validated

CKAP2L Polyclonal antibody

CKAP2L Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17143-1-AP

Synonyms

CKAP2L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKAP2L Polyclonal antibody (17143-1-AP)

    WB analysis using 17143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CKAP2L Polyclonal antibody (17143-1-AP)

    WB analysis using 17143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CKAP2L Polyclonal antibody (17143-1-AP)

    WB analysis of K-562 using 17143-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CKAP2L Polyclonal antibody (17143-1-AP)

    IP experiment of Jurkat using 17143-1-AP

    IP result of anti-CKAP2L (IP:17143-1-AP, 4ug; Detection:17143-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, Jurkat cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17143-1-AP targets CKAP2L in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP2L fusion protein Ag10909
Full Name cytoskeleton associated protein 2-like
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC036217
Gene Symbol CKAP2L
Gene ID (NCBI) 150468
RRIDAB_2080720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CKAP2L antibody 17143-1-APDownload protocol
IP protocol for CKAP2L antibody 17143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Clin Exp Pathol

Oncogenic and prognostic role of CKAP2L in hepatocellular carcinoma.

Authors - Penghui Wang
  • KD Validated
View Article
humanWB,IHC

FASEB J

IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6 A-dependent manner

Authors - Yaqian Shi
View Article