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  • Featured Product
  • KD/KO Validated

CLASP2 Polyclonal antibody

CLASP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12942-1-AP

Synonyms

CLASP2, CLIP associating protein 2, hOrbit2, KIAA0627, Protein Orbit homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using CLASP2 Polyclonal antibody (12942-1-AP)

    WB analysis of human brain using 12942-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CLASP2 Polyclonal antibody (12942-1-AP)

    WB analysis of HEK-293 using 12942-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLASP2 Polyclonal antibody (12942-1-AP)

    IHC staining of mouse brain using 12942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLASP2 Polyclonal antibody (12942-1-AP)

    IHC staining of mouse brain using 12942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLASP2 Polyclonal antibody (12942-1-AP)

    IF Staining of HepG2 using 12942-1-AP

    Immunofluorescent analysis of HepG2 cells using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CLASP2 Polyclonal antibody (12942-1-AP)

    IF Staining of mouse brain using 12942-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CLASP2 Polyclonal antibody (12942-1-AP)

    IF Staining of mouse brain using 12942-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12942-1-AP targets CLASP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLASP2 fusion protein Ag4012
Full Name cytoplasmic linker associated protein 2
Calculated Molecular Weight 1294 aa, 141 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC029035
Gene Symbol CLASP2
Gene ID (NCBI) 23122
RRIDAB_2260448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Protocols

Product Specific Protocols
WB protocol for CLASP2 antibody 12942-1-APDownload protocol
IHC protocol for CLASP2 antibody 12942-1-APDownload protocol
IF protocol for CLASP2 antibody 12942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Bone Miner Res

Regulation of bone resorption and sealing zone formation in osteoclasts occurs through protein kinase B-mediated microtubule stabilization.

Authors - Matsumoto Takumi T
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sarah (Verified Customer) (07-06-2020)

30ug HEK protein loaded, and CLASP2 levels detected for control cells or cells transfected with CLASP2 siRNA

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK 293T
CLASP2 Antibody Western Blot validation (1:500 dilution) in HEK 293T (Cat no:12942-1-AP)