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WB analysis using 14402-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 14402-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14402-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 14402-1-AP
IP result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.
IHC staining of human kidney using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 14402-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

CLCNKA Polyclonal antibody

Name: CLCNKA Polyclonal antibody
Brand: Proteintech
SKU: 14402-1-AP

CLCNKA Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14402-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse kidney tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14402-1-AP targets CLCNKA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5647 Product name: Recombinant human CLCNKA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 515-687 aa of BC048282 Sequence: CQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK Predict reactive species
Full Name	chloride channel Ka
Calculated Molecular Weight	75 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC048282
Gene Symbol	CLCNKA
Gene ID (NCBI)	1187
RRID	AB_2079857
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51800
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLCNKA antibody 14402-1-AP	Download protocol
IHC protocol for CLCNKA antibody 14402-1-AP	Download protocol
IP protocol for CLCNKA antibody 14402-1-AP	Download protocol