Tested Applications
| Positive WB detected in | mouse brain tissue, K-562 cells, mouse spleen tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26077-1-AP targets CLEC12B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23396 Product name: Recombinant human CLEC12B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 65-232 aa of BC136805 Sequence: LQISNDINSDSEKLSQLQKTIQQQQDNLSQQLGNSNNLSMEEEFLKSQISSLLKRQEQMAIKLCQELIIHTSDHRCNPCPKMWQWYQNSCYYFTTNEEKTWANSRKDCIDKNSTLVKIDSLEEKDFLMSQPLLMFSFFWLGLSWDSSGRSWFWEDGSVPSPSLYVSNY Predict reactive species |
| Full Name | C-type lectin domain family 12, member B |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC136805 |
| Gene Symbol | CLEC12B |
| Gene ID (NCBI) | 387837 |
| RRID | AB_2880365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2HXU8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLEC12B (C-type lectin domain family 12 member B) is a single-pass type II membrane protein of the C-type lectin-like family. CLEC12B acts as an inhibitory receptor on myeloid cells. CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. (PMID: 17562706)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC12B antibody 26077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
