Tested Applications
| Positive WB detected in | mouse testis tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14746-1-AP targets CLP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6479 Product name: Recombinant human CLP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 123-425 aa of BC000446 Sequence: TDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK Predict reactive species |
| Full Name | CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 41 kDa, 48 kDa |
| GenBank Accession Number | BC000446 |
| Gene Symbol | CLP1 |
| Gene ID (NCBI) | 10978 |
| RRID | AB_2878076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92989 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLP1 antibody 14746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Mol Cell m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (09-12-2025) | Consistently detects the target protein in WB. Tested on HeLa total cell extracts
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