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WB analysis using 11766-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11766-1-AP (CLPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of rat pancreas using 11766-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 11766-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 11766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 11766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse pancreas using 11766-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (11766-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLPS Polyclonal antibody

Name: CLPS Polyclonal antibody
Brand: Proteintech
SKU: 11766-1-AP

CLPS Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11766-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Colipase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse pancreas tissue, rat pancreas tissue
Positive IHC detected in	rat pancreas tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse pancreas tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11766-1-AP targets CLPS in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2321 Product name: Recombinant human CLPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-112 aa of BC017897 Sequence: MEKILILLLVALSVAYAAPGPRGIIINLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGRSKQ Predict reactive species
Full Name	colipase, pancreatic
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12 kDa
GenBank Accession Number	BC017897
Gene Symbol	CLPS
Gene ID (NCBI)	1208
RRID	AB_10863117
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04118
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

Protocols

Product Specific Protocols
IF protocol for CLPS antibody 11766-1-AP	Download protocol
IHC protocol for CLPS antibody 11766-1-AP	Download protocol
WB protocol for CLPS antibody 11766-1-AP	Download protocol