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- KD/KO Validated
CMIP Polyclonal antibody
CMIP Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12851-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, Raji cells |
| Positive IP detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
12851-1-AP targets CMIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CMIP fusion protein Ag3475 |
| Full Name | c-Maf-inducing protein |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC038113 |
| Gene Symbol | CMIP |
| Gene ID (NCBI) | 80790 |
| RRID | AB_2081366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CMIP antibody 12851-1-AP | Download protocol |
| IP protocol for CMIP antibody 12851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal Inhibition of nephrin activation by c-mip through Csk-Cbp-Fyn axis plays a critical role in Angiotensin II-induced podocyte damage.
| ||
Cell Physiol Biochem Induction of C-Mip by IL-17 Plays an Important Role in Adriamycin-Induced Podocyte Damage. | ||
Mol Med Rep c‑Maf inducing protein inhibits cofilin‑1 activity and alters podocyte cytoskeleton organization. | ||
