Tested Applications
| Positive WB detected in | HepG2 cells, LNCaP cells, mouse kidney tissue, rat kidney rat kidney |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 14 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
14925-1-AP targets CNDP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6684 Product name: Recombinant human CNDP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-475 aa of BC001375 Sequence: KLYGRGSTDDKGPVAGWINALEAYQKTGQEIPVNVRFCLEGMEESGSEGLDELIFARKDTFFKDVDYVCISDNYWLGKKKPCITYGLRGICYFFIEVECSNKDLHSGVYGGSVHEAMTDLILLMGSLVDKRGNILIPGINEAVAAVTEEEHKLYDDIDFDIEEFAKDVGAQILLHSHKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPNMTPEVVGEQVTSYLTKKFAELRSPNEFKVYMGHGGKPWVSDFSHPHYLAGRRAMKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRYNYIEGTKMLAAYLYEVSQLKD Predict reactive species |
| Full Name | CNDP dipeptidase 2 (metallopeptidase M20 family) |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 44-53 kDa |
| GenBank Accession Number | BC001375 |
| Gene Symbol | CNDP2 |
| Gene ID (NCBI) | 55748 |
| RRID | AB_2081682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KP4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa.
The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| IHC protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| IP protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| WB protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease. | ||
Cell Chem Biol Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.
| ||
Mol Med Underexpressed CNDP2 participates in gastric cancer growth inhibition through activating the MAPK signaling pathway. | ||
BMC Cancer The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study | ||
PLoS One Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma. | ||
BMC Gastroenterol Up-regulation of CNDP2 facilitates the proliferation of colon cancer.
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