Tested Applications
| Positive WB detected in | Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 12 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
11135-1-AP targets CNOT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1587 Product name: Recombinant human CNOT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-753 aa of BC016474 Sequence: NASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ Predict reactive species |
| Full Name | CCR4-NOT transcription complex, subunit 3 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC016474 |
| Gene Symbol | CNOT3 |
| Gene ID (NCBI) | 4849 |
| RRID | AB_2229682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75175 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| WB protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| IF protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.
| ||
Nat Cancer ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1. | ||
bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Maria (Verified Customer) (10-09-2025) | It works perfectly for detection in WB and for IP at 4ug/IP for 600 ug of protein lysate.
|
FH Roy (Verified Customer) (06-13-2024) | Great CNOT3 bands by WB in HeLa cells (1/1000 dilution, overnight incubation at 4°C).
|
FH Hannah (Verified Customer) (02-06-2019) | Works well and specifically for WB on human fibroblast lysates.
 |
