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WB analysis of Jurkat using 10752-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10752-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10752-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10752-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 10752-1-AP
IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug.
IHC staining of human prostate cancer using 10752-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human prostate cancer using 10752-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 10752-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 10752-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10752-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10752-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT8 antibody (10752-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

CNOT8 Polyclonal antibody

Name: CNOT8 Polyclonal antibody
Brand: Proteintech
SKU: 10752-1-AP
Rating: 4.50 (2 reviews)

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10752-1-AP

Publications(7)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (7)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human prostate cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IF	See 1 publications below

Product Information

10752-1-AP targets CNOT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1201 Product name: Recombinant human CNOT8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-292 aa of BC017366 Sequence: MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQ Predict reactive species
Full Name	CCR4-NOT transcription complex, subunit 8
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC017366
Gene Symbol	CNOT8
Gene ID (NCBI)	9337
RRID	AB_2082470
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UFF9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

Protocols

Product Specific Protocols
IF protocol for CNOT8 antibody 10752-1-AP	Download protocol
IHC protocol for CNOT8 antibody 10752-1-AP	Download protocol
IP protocol for CNOT8 antibody 10752-1-AP	Download protocol
WB protocol for CNOT8 antibody 10752-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation Authors - Markus Höpfler KD Validated View Article
human	WB	Mol Biosyst Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae. Authors - Samanfar Bahram B KD Validated View Article
mouse	WB	Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). Authors - Tian-Li Duan View Article
human	WB	Nucleic Acids Res BESST: a novel LncRNA knockout strategy with less genome perturbance Authors - Shikuan Zhang View Article
human	WB,IF	Cells Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability Authors - Nafiseh Chalabi Hagkarim KD Validated View Article
	WB	Nat Commun Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes Authors - Yusheng Liu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Aditya (Verified Customer) (10-08-2024)	works okay, better than cnot9 Applications: Western Blot
FH Roy (Verified Customer) (06-12-2024)	Works well to detect CNOT8 by Western Blot (1/500 Dilution) Applications: Western Blot Primary Antibody Dilution: 1/500 Cell Tissue Type: HeLa