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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 4 publications below |
Product Information
26880-1-AP targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25454 Product name: Recombinant human CNTROB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC021134 Sequence: MATSADSPSSPLGAEDLLSDSSEPPGLNQVSSEVTSQLYASLRLSRQAEATARAQLYLPSTSPPHEGLDGFAQELSRSLSVGLEKNLKKKDGSKHIFEMESVRGQLQTMLQTSRDTAYRDPLIPGAGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPLNPPDFQGLRDALDSEHTRRKHCERHIQSLQTRVLELQQQLAVAVAADRKKDTMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEEHQAAELTRSKQQETVTRLEQSLSEAMEALNREQESARLQQRERETLEEERQALTLRLEAEQQRCCVLQEERDAARAGQLSEHRELET Predict reactive species |
| Full Name | centrobin, centrosomal BRCA2 interacting protein |
| Calculated Molecular Weight | 903 aa, 101 kDa |
| Observed Molecular Weight | 60-70 kDa, 100-130 kDa |
| GenBank Accession Number | BC021134 |
| Gene Symbol | CNTROB |
| Gene ID (NCBI) | 116840 |
| RRID | AB_2880667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTROB antibody 26880-1-AP | Download protocol |
| IF protocol for CNTROB antibody 26880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol The central scaffold protein CEP350 coordinates centriole length, stability, and maturation | ||
Elife Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number. | ||
Mol Biol Cell The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis. | ||
