Filter: AllWBIHCIF/ICC

WB analysis of human brain using 14277-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 14277-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14277-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 14277-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14277-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14277-1-AP
Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 14277-1-AP
Immunofluorescent analysis of Hela cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

COG6 Polyclonal antibody

Name: COG6 Polyclonal antibody
Brand: Proteintech
SKU: 14277-1-AP

COG6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14277-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COD2, COG complex subunit 6, KIAA1134

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14277-1-AP targets COG6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5624 Product name: Recombinant human COG6 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 308-657 aa of BC051723 Sequence: VGDMLAWLHQATASEKEHLEALLKHVTTQGVEENIQEVVGHITEGVCRPLKVRIEQVIVAEPGAVLLYKISNLLKFYHHTISGIVGNSATALLTTIEEMHLLSKKIFFNSLSLHASKLMDKVELPPPDLGPSSALNQTLMLLREVLASHDSSVVPLDARQADFVQVLSCVLDPLLQMCTVSASNLGTADMATFMVNSLYMMKTTLALFEFTDRRLEMLQFQIEAHLDTLINEQASYVLTRVGLSYIYNTVQQHKPEQGSLANMPNLDSVTLKAAMVQFDRYLSAPDNLLIPQLNFLLSATVKEQIVKQSTELVCRAYGEVYAAVMNPINEYKDPENILHRSPQQVQTLLS Predict reactive species
Full Name	component of oligomeric golgi complex 6
Calculated Molecular Weight	73 kDa
Observed Molecular Weight	73 kDa
GenBank Accession Number	BC051723
Gene Symbol	COG6
Gene ID (NCBI)	57511
RRID	AB_10694426
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2V7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for COG6 antibody 14277-1-AP	Download protocol
IHC protocol for COG6 antibody 14277-1-AP	Download protocol
WB protocol for COG6 antibody 14277-1-AP	Download protocol