COIL Antibody
Rabbit Polyclonal
Catalog number: 10967-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | human brain tissue, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP(COIL Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 10967-1-AP(COIL Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using COIL antibody 10967-1-AP at 1:50 and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | COIL fusion protein ag1416 | Full name | coilin |
|
Calculated molecular weight |
62.6kd |
Observed molecular
weight |
80kd |
|
GenBank accession number |
BC010385 | Gene ID (NCBI) | 8161 |
| Gene symbol | COIL | Synonyms | CLN80; p80-coilin |
Background
The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs |
