Collagen Type XXIII Polyclonal antibody

Collagen Type XXIII Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14337-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

COL23, COL23A1, Collagen alpha 1(XXIII) chain, Collagen alpha-1(XXIII) chain, collagen, type XXIII, alpha 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis of mouse thymus using 14337-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse thymus using 14337-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 14337-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 14337-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	mouse thymus tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14337-1-AP targets Collagen Type XXIII in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5640 Product name: Recombinant human COL23A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-309 aa of BC042428 Sequence: MESRSGIQAGVRCRDLGSLQPPPLALKQFSSLSLPSSWDYRRLPPRCGFLFEVSETTNPPAGTNSRHTLTVKLDGLAKIRTAREAPSECVCPPGPPGRRGKPGRRGDPGPPGPLGLDGKPGLPGPKGEKGAPGDFGPRGDQGQDGAAGPPGPPGPPGARGPPGDTGKDGPRGAQGPAGPKGEPGQDGEMGPKGPPGPKGEPGVPGKKGDDGTPSQPGPPGPKGEPGSMGPRGENGVDGAPGPKGEPGHRGTDGAAGPRGDVRDPGLGSVSSCSQRLASSSKKNGSEPPPGCAGCPRPQGRAGRHSGDRL Predict reactive species
Full Name	collagen, type XXIII, alpha 1
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	68-75 kDa
GenBank Accession Number	BC042428
Gene Symbol	COL23A1
Gene ID (NCBI)	91522
RRID	AB_2276392
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86Y22
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.